Inhibition of in-vitro growth of Plasmodium falciparum by Pseudocedrela kotschyi extract alone and in combination with Fagara zanthoxyloides extract
Identifieur interne : 001788 ( Main/Exploration ); précédent : 001787; suivant : 001789Inhibition of in-vitro growth of Plasmodium falciparum by Pseudocedrela kotschyi extract alone and in combination with Fagara zanthoxyloides extract
Auteurs : Olakunle O. Kassim [États-Unis] ; Mark Loyevsky [États-Unis] ; Henrietta Amonoo [États-Unis] ; Liesel Lashley [États-Unis] ; Kwashie A. Ako-Nai [Nigeria] ; Victor R. Gordeuk [États-Unis]Source :
- Transactions of The Royal Society of Tropical Medicine and Hygiene [ 0035-9203 ] ; 2009.
Abstract
Roots of Pseudocedrela kotschyi are commonly used as chewing sticks in West Africa. This study examined the effects of the plant extract on the in-vitro growth of Plasmodium falciparum. Ring-stage synchronised cultures of the malaria parasite were exposed to 30 and 60 μg/ml of P. kotschyi extract for 51 h. Aliquots were taken from the cultures every 3 h for preparation of Giemsa-stained thin films, which were evaluated by light microscopy for degree of parasitaemia and stage distribution of parasite development. The extracts did not show any inhibitory effects on the emergence of trophozoites in treated cultures. However, the results indicate that 80% of inhibition of the parasite transformation into schizont was obtained for both tested concentrations (30 and 60 μg/ml). Experiments with 3H-hypoxanthine incorporation showed an IC50 of 16 μg/ml for the Pseudocedrela extract. Pseudocedrela was combined with extract of Fagara zanthoxyloides in various concentrations to determine their interactive effects on the in-vitro cultures. Isobologram analysis of the results indicated a synergistic interaction between the two extracts at low concentrations, while interactions at higher concentrations showed antagonistic effects.
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DOI: 10.1016/j.trstmh.2009.02.018
Affiliations:
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This study examined the effects of the plant extract on the in-vitro growth of Plasmodium falciparum. Ring-stage synchronised cultures of the malaria parasite were exposed to 30 and 60 μg/ml of P. kotschyi extract for 51 h. Aliquots were taken from the cultures every 3 h for preparation of Giemsa-stained thin films, which were evaluated by light microscopy for degree of parasitaemia and stage distribution of parasite development. The extracts did not show any inhibitory effects on the emergence of trophozoites in treated cultures. However, the results indicate that 80% of inhibition of the parasite transformation into schizont was obtained for both tested concentrations (30 and 60 μg/ml). Experiments with 3H-hypoxanthine incorporation showed an IC50 of 16 μg/ml for the Pseudocedrela extract. Pseudocedrela was combined with extract of Fagara zanthoxyloides in various concentrations to determine their interactive effects on the in-vitro cultures. Isobologram analysis of the results indicated a synergistic interaction between the two extracts at low concentrations, while interactions at higher concentrations showed antagonistic effects.</div></front></TEI><affiliations><list><country><li>Nigeria</li><li>États-Unis</li></country><region><li>District de Columbia</li></region></list><tree><country name="États-Unis"><region name="District de Columbia"><name sortKey="Kassim, Olakunle O" sort="Kassim, Olakunle O" uniqKey="Kassim O" first="Olakunle O." last="Kassim">Olakunle O. Kassim</name></region><name sortKey="Amonoo, Henrietta" sort="Amonoo, Henrietta" uniqKey="Amonoo H" first="Henrietta" last="Amonoo">Henrietta Amonoo</name><name sortKey="Gordeuk, Victor R" sort="Gordeuk, Victor R" uniqKey="Gordeuk V" first="Victor R." last="Gordeuk">Victor R. Gordeuk</name><name sortKey="Gordeuk, Victor R" sort="Gordeuk, Victor R" uniqKey="Gordeuk V" first="Victor R." last="Gordeuk">Victor R. Gordeuk</name><name sortKey="Kassim, Olakunle O" sort="Kassim, Olakunle O" uniqKey="Kassim O" first="Olakunle O." last="Kassim">Olakunle O. Kassim</name><name sortKey="Lashley, Liesel" sort="Lashley, Liesel" uniqKey="Lashley L" first="Liesel" last="Lashley">Liesel Lashley</name><name sortKey="Loyevsky, Mark" sort="Loyevsky, Mark" uniqKey="Loyevsky M" first="Mark" last="Loyevsky">Mark Loyevsky</name></country><country name="Nigeria"><noRegion><name sortKey="Ako Nai, Kwashie A" sort="Ako Nai, Kwashie A" uniqKey="Ako Nai K" first="Kwashie A." last="Ako-Nai">Kwashie A. Ako-Nai</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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